Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci | Zendy

Jason Prystowsky | Zendy; Farida Siddiqui | Zendy; John G. Chosay | Zendy; Dean L. Shinabarger | Zendy; J Gordon Millichap | Zendy; Lance R. Peterson | Zendy; Gary A. Noskin | Zendy

Open Access

Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci

Author(s) -

Jason Prystowsky,

Farida Siddiqui,

John G. Chosay,

Dean L. Shinabarger,

J Gordon Millichap,

Lance R. Peterson,

Gary A. Noskin

Publication year - 2001

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.45.7.2154-2156.2001

Subject(s) - linezolid , 23s ribosomal rna , vancomycin resistant enterococci , microbiology and biotechnology , vancomycin , enterococcus , gram positive bacterial infections , biology , genetics , antibiotics , bacteria , staphylococcus aureus , gene , rna , ribosome

To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, five Enterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E. faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 microg/ml contained a guanine to adenine transition at bp 2505.

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