Open AccessRestoration of Vancomycin Susceptibility in Enterococcus faecalis by Antiresistance Determinant Gene Transfer
Author(s) -
C. Torres Viera,
Sotirios Tsiodras,
Howard S. Gold,
Eoin Coakley,
Christine Wennersten,
George M. Eliopoulos,
Robert C. Moellering,
Roger T. Inouye
Publication year - 2001
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.45.3.973-975.2001
Subject(s) - enterococcus faecalis , gene transfer , vancomycin , enterococcus , microbiology and biotechnology , biology , gene , antibacterial agent , streptococcaceae , antibiotics , genetics , bacteria , staphylococcus aureus , escherichia coli
We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.
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