Open AccessChloramphenicol-Sensitive Escherichia coli Strain Expressing the Chloramphenicol Acetyltransferase ( cat ) Gene
Author(s) -
Joanna Potrykus,
Grzegorz Węgrzyn
Publication year - 2001
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.45.12.3610-3612.2001
Subject(s) - chloramphenicol acetyltransferase , chloramphenicol , escherichia coli , acetyltransferase , strain (injury) , microbiology and biotechnology , biology , gene , acetyltransferases , antibiotics , reporter gene , gene expression , biochemistry , acetylation , anatomy
An Escherichia coli strain (strain CM2555) bearing the chloramphenicol acetyltransferase (cat) gene was found to be sensitive to chloramphenicol. We demonstrate that the cat gene is efficiently expressed in strain CM2555. Our results suggest that decreased levels of acetyl coenzyme A in cat-expressing CM2555 cells in the presence of chloramphenicol may cause the bacterium to be sensitive to this antibiotic.