Open AccessAmino Acid Substitutions in a Variant of IMP-1 Metallo-β-Lactamase
Author(s) -
Shizuko Iyobe,
Haruko Kusadokoro,
Junko Ozaki,
Naoki Matsumura,
Shinji Minami,
Shin Haruta,
Tetsuo Sawai,
Koji Ohara
Publication year - 2000
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.44.8.2023-2027.2000
Subject(s) - biology , gene , nucleotide , integron , nucleic acid sequence , biochemistry , benzylpenicillin , serine , microbiology and biotechnology , plasmid , genetics , enzyme , penicillin , antibiotics
In the course of surveying for the carbapenem-hydrolyzing metallo-β-lactamase genebla IMP in pathogenic bacteria by the PCR method, we detected a gene encoding a variant metallo-β-lactamase, designated IMP-3, which differed from IMP-1 by having low hydrolyzing activity for penicillins and carbapenems. PCR product direct sequencing of a 2.2-kb segment revealed that the genebla IMP-3 was located on a cassette inserted within a class I integron in the pMS390 plasmid. The 741-bp nucleotide sequence ofbla IMP-3 was identical to that ofbla IMP-1 , except for seven base substitutions. Among these were two, at nucleotide positions 314 and 640, which caused amino acid alterations. Hybridbla genes were constructed frombla IMP-3 andbla IMP-1 by recombinant DNA techniques, and β-lactamases encoded by these genes were compared with those of the parents IMP-3 and IMP-1 under the same experimental conditions. The kinetic parameters indicated that the inefficient hydrolysis of benzylpenicillin, ampicillin, imipenem, and ceftazidime by IMP-3 was due to the substitution of glycine for serine at amino acid residue 196 in the mature enzyme. This alteration corresponded to the presence of guanine instead of an adenine at nucleotide position 640 of thebla IMP-3 gene. This indicated that extension of the substrate profile in the metallo-β-lactamase IMP-1 compared to IMP-3 is the result of a one-step single-base mutation, suggesting that the genebla IMP-3 is an ancestor ofbla IMP-1 .