Open AccessCloning, Nucleotide Sequencing, and Analysis of the Gene Encoding an AmpC β-Lactamase in Acinetobacter baumannii
Author(s) -
Germán Bou,
J Martı́nez-Beltrán
Publication year - 2000
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.44.2.428-432.2000
Subject(s) - acinetobacter baumannii , plasmid , biology , gene , microbiology and biotechnology , acinetobacter , nucleic acid sequence , strain (injury) , antibiotics , sequence analysis , genetics , bacteria , pseudomonas aeruginosa , anatomy
A clinical strain ofAcinetobacter baumannii (strain Ab RYC 52763/97) that was isolated during an outbreak in our hospital and that was resistant to all β-lactam antibiotics tested produced three β-lactamases: a TEM-1-type (pI, 5.4) plasmid-mediated β-lactamase, a chromosomally mediated OXA-derived (pI, 9.0) β-lactamase, and a presumptive chromosomal cephalosporinase (pI, 9.4). The nucleotide sequence of the chromosomal cephalosporinase gene shows for the first time the gene encoding an AmpC β-lactamase inA. baumannii . In addition, we report here the biochemical properties of thisA. baumannii AmpC β-lactamase.