Relationship between Pyrazinamide Resistance, Loss of Pyrazinamidase Activity, and Mutations in the pncA Locus in Multidrug-Resistant Clinical Isolates of Mycobacterium tuberculosis | Zendy

Marian Mestdagh | Zendy; Pierre-Alain Fonteyne | Zendy; L. Realini | Zendy; R. Rossau | Zendy; Geert Jannes | Zendy; W. Mijs | Zendy; Koen A. L. De Smet | Zendy; Françoise Portaels | Zendy; E. Van den Eeckhout | Zendy

Open Access

Relationship between Pyrazinamide Resistance, Loss of Pyrazinamidase Activity, and Mutations in the pncA Locus in Multidrug-Resistant Clinical Isolates of Mycobacterium tuberculosis

Author(s) -

Marian Mestdagh,

Pierre-Alain Fonteyne,

L. Realini,

R. Rossau,

Geert Jannes,

W. Mijs,

Koen A. L. De Smet,

Françoise Portaels,

E. Van den Eeckhout

Publication year - 1999

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.43.9.2317

Subject(s) - pyrazinamide , mycobacterium tuberculosis , microbiology and biotechnology , biology , multiple drug resistance , tuberculosis , gene , locus (genetics) , genetics , drug resistance , antibiotics , rifampicin , medicine , pathology

Sixty-two Mycobacterium tuberculosis isolates were tested for pyrazinamidase activity, and their pyrazinamide susceptibility was determined by the radiometric method. Sequencing of pncA genes in the 23 resistant strains revealed mutations in 16 pyrazinamidase-negative strains, 11 of which had not been previously described. Six isolates containing wild-type pncA might possess alternative resistance mechanisms.

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