Rapid Detection, by PCR and Reverse Hybridization, of Mutations in the Helicobacter pylori 23S rRNA Gene, Associated with Macrolide Resistance | Zendy

LeenJan van Doorn | Zendy; Y. J. Debets-Ossenkopp | Zendy; Armelle Marais | Zendy; Ricardo Sanna | Zendy; Françis Mégraud | Zendy; Johannes G. Kusters | Zendy; Wim Quint | Zendy

Open Access

Rapid Detection, by PCR and Reverse Hybridization, of Mutations in the Helicobacter pylori 23S rRNA Gene, Associated with Macrolide Resistance

Author(s) -

LeenJan van Doorn,

Y. J. Debets-Ossenkopp,

Armelle Marais,

Ricardo Sanna,

Françis Mégraud,

Johannes G. Kusters,

Wim Quint

Publication year - 1999

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.43.7.1779

Subject(s) - 23s ribosomal rna , biology , helicobacter pylori , point mutation , microbiology and biotechnology , ribosomal rna , restriction fragment length polymorphism , gene , polymerase chain reaction , nucleic acid thermodynamics , dna , mutant , genetics , rna , ribosome

A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations, associated with macrolide resistance in Helicobacter pylori. Fifty-seven H. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by PCR-LiPA (detecting mutations A2115-->G, G2141-->A, A2142-->G, A2142-->C, A2143-->G, A2143-->C, and A2143-->T), DNA sequencing, restriction fragment length polymorphism, and/or hybridization to oligonucleotide probes. Results were highly concordant, but PCR-LiPA appears to be more sensitive for the simultaneous detection of multiple mutants.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research