Open AccessNew Trimethoprim-Resistant Dihydrofolate Reductase Cassette, dfrXV , Inserted in a Class 1 Integron
Author(s) -
Peter V. Adrian,
Mig du Plessis,
Keith P. Klugman,
Sebastian G. B. Amyes
Publication year - 1998
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.42.9.2221
Subject(s) - integron , dihydrofolate reductase , biology , plasmid , gene , gene cassette , genetics , nucleic acid sequence , spectinomycin , escherichia coli , microbiology and biotechnology , tetracycline , antibiotics
The nucleotide sequence of a plasmid-borne trimethoprim resistance gene from a commensal fecal Escherichia coli isolate revealed a new dihydrofolate reductase gene, dfrXV, which occurred as a gene cassette integrated in a site-specific manner in a class 1 integron. The new gene shows 84% nucleotide identity and the predicted protein shows 90% amino acid identity with dfrI and DHFR type I, respectively. Genes for spectinomycin resistance, aadA1 [ant (3")-Ia], and sulfonamide resistance, sulI, were located downstream of dfrXV in a manner identical to that in pLMO229.
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