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DNA sequences of three 18S rRNA gene alleles present in trophozoites obtained before and after therapy for Acanthamoeba keratitis substantiate a previous report that the infection was due to a single Acanthamoeba strain. Thus, the possibility that propamidine resistance which developed during therapy was due to a mixed infection was ruled out.

antimicrobial agents and chemotherapy

Confirmatory Evidence from 18S rRNA Gene Analysis for In Vivo Development of Propamidine Resistance in a Temporal Series of <i>Acanthamoeba</i> Ocular Isolates from a Patient

Confirmatory Evidence from 18S rRNA Gene Analysis for In Vivo Development of Propamidine Resistance in a Temporal Series of Acanthamoeba Ocular Isolates from a Patient