Alginate Lyase Promotes Diffusion of Aminoglycosides through the Extracellular Polysaccharide of Mucoid Pseudomonas aeruginosa | Zendy

Richard A. Hatch | Zendy; Neal L. Schiller | Zendy

Open Access

Alginate Lyase Promotes Diffusion of Aminoglycosides through the Extracellular Polysaccharide of Mucoid Pseudomonas aeruginosa

Author(s) -

Richard A. Hatch,

Neal L. Schiller

Publication year - 1998

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.42.4.974

Subject(s) - tobramycin , pseudomonas aeruginosa , carbenicillin , microbiology and biotechnology , aminoglycoside , gentamicin , cystic fibrosis , chemistry , extracellular , antibiotics , bacteria , biology , biochemistry , genetics

We demonstrated that a 2% suspension ofPseudomonas aeruginosa alginate completely blocked the diffusion of gentamicin and tobramycin, but not that of carbenicillin, illustrating how alginate production can help protectP. aeruginosa growing within alginate microcolonies in patients with cystic fibrosis (CF) from the effects of aminoglycosides. This aminoglycoside diffusion barrier was degraded with a semipurified preparation ofP. aeruginosa alginate lyase, suggesting that this enzyme deserves consideration as an adjunctive agent for CF patients colonized by mucoid strains ofP. aeruginosa.

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