Chromogenic Detection of Aminoglycoside Phosphotransferases | Zendy

Ana Amoroso | Zendy; Gabriel Gutkind | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Chromogenic Detection of Aminoglycoside Phosphotransferases

Author(s) -

Ana Amoroso,

Gabriel Gutkind

Publication year - 1998

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.42.2.228

Subject(s) - phosphoenolpyruvate carboxykinase , kanamycin , aminoglycoside , phosphotransferases , biochemistry , pyruvate kinase , chromogenic , chemistry , microbiology and biotechnology , biology , enzyme , antibiotics , glycolysis , chromatography

A coupled chromogenic reaction (based on an agar overlay combining NADH, pyruvate kinase, lactate dehydrogenase, phosphoenolpyruvate, ATP, and kanamycin sulfate with thiazolyl blue-phenazine methosulfate for detection of NADH consumption) was optimized for the detection of aminoglycoside phosphotransferases (APHs). When used after analytical isoelectrofocusing of bacterial extracts from APH-producing strains, this method revealed one band in each of two strains with a genetically confirmed APH (3') I and two bands in another strain with both APH (3') I and APH (3') VI, whereas no bands were detected in susceptible control strains or in aminoglycoside-resistant microorganisms without APH genes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research