Vancomycin dependence in a vanA-producing Enterococcus avium strain with a nonsense mutation in the natural D-Ala-D-Ala ligase gene

FIG. 1. Amino acid sequences deduced from the amplified fragments of strains Ea1, Ea3, and Ea31. The amino acid sequence of the reference strain E. avium ATTC 14025 is shown. Only the stretches which have been determined in common (reference 2 and the present work) are shown. The numbering system was taken from that for the Escherichia coli DdlB ligase as described previously (2); the sequence VVKE, present in all E. avium strains but not in the E. coli ligase, was therefore not counted and is shown in brackets. Bold letters represent conserved amino acids. Positions 121, 186, 245, 246, and 269 are those where amino acid differences were found. Asterisks represent the position of the stop codon.