Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila | Zendy

Thomas Suter | Zendy; V. K. Viswanathan | Zendy; Nicholas P. Cianciotto | Zendy

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Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila

Author(s) -

Thomas Suter,

V. K. Viswanathan,

Nicholas P. Cianciotto

Publication year - 1997

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.41.6.1385

Subject(s) - spectinomycin , legionella pneumophila , kanamycin , microbiology and biotechnology , biology , phosphotransferase , legionnaires' disease , legionella , aminocyclitol , aminoglycoside , gene , streptomycin , virology , genetics , bacteria , antibiotics

A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.

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