Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra | Zendy

C J Hackbarth | Zendy; İbrahim Ünsal | Zendy; Henry F. Chambers | Zendy

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Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra

Author(s) -

C J Hackbarth,

İbrahim Ünsal,

Henry F. Chambers

Publication year - 1997

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.41.5.1182

Subject(s) - mycobacterium tuberculosis , cefoxitin , mycobacterium smegmatis , recombinant dna , cosmid , biology , cloning (programming) , microbiology and biotechnology , sequence analysis , virology , plasmid , tuberculosis , genetics , gene , bacteria , medicine , pathology , computer science , programming language , staphylococcus aureus

A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.

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