Use of Mono Mac 6 human monocytic cell line and J774 murine macrophage cell line in parallel antimycobacterial drug studies | Zendy

E L Wright | Zendy; Debra C. Quenelle | Zendy; William J. Suling | Zendy; William W. Barrow | Zendy

Open Access

Use of Mono Mac 6 human monocytic cell line and J774 murine macrophage cell line in parallel antimycobacterial drug studies

Author(s) -

E L Wright,

Debra C. Quenelle,

William J. Suling,

William W. Barrow

Publication year - 1996

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.40.9.2206

Subject(s) - antimycobacterial , intracellular , cell culture , macrophage , mycobacterium tuberculosis , drug , intracellular parasite , microbiology and biotechnology , biology , tuberculosis , cell , mycobacterium , monocyte , immunology , virology , pharmacology , medicine , in vitro , pathology , biochemistry , genetics

The Mono Mac 6 (MM6) human monocytic cell line was evaluated with the established J774 murine macrophage cell line to ascertain its effectiveness in determining the intracellular activities of antimycobacterial drugs. Cells were infected with Mycobacterium tuberculosis H37Ra and treated with drug concentrations corresponding to the MICs, as well as to threefold higher than and threefold less than the MICs. Changes in CFU were compared after 7 days to determine significant differences between treated and nontreated groups. The results suggest that MM6 will make a useful model for testing the intracellular activities of antituberculosis drugs.

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