Comparative evaluation of microplate enzyme-linked immunosorbent assay versus plaque reduction assay for antiviral susceptibility testing of herpes simplex virus isolates | Zendy

Sharon Safrin | Zendy; E Palacios | Zendy; Bradley J. Leahy | Zendy

Open Access

Comparative evaluation of microplate enzyme-linked immunosorbent assay versus plaque reduction assay for antiviral susceptibility testing of herpes simplex virus isolates

Author(s) -

Sharon Safrin,

E Palacios,

Bradley J. Leahy

Publication year - 1996

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.40.4.1017

Subject(s) - foscarnet , herpes simplex virus , virology , virus quantification , virus , alphaherpesvirinae , concordance , simplexvirus , herpesviridae , herpes genitalis , biology , viral disease , genital herpes , bioinformatics

We tested the antiviral susceptibilities of 30 clinical isolates of herpes simplex virus using the microplate in situ enzyme-linked immunosorbent assay (MISE) and the plaque reduction assay (PRA). There was concordance for 26 of 30 acyclovir results and all 30 foscarnet results. MISE and PRA results each predicted the response to acyclovir in 12 of 14 instances and the response to foscarnet in 8 instances. MISE is more rapid than PRA, has an objective endpoint, and correlates well with the clinical response to therapy.

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