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Dialysis culture enables more accurate determination of MIC of benzylpenicillin for Borrelia burgdorferi than does conventional procedure
Author(s) -
Susanne Hammers Stiernstedt,
Bengt Wretlind
Publication year - 1996
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.40.12.2882
Subject(s) - benzylpenicillin , penicillin , borrelia burgdorferi , microbiology and biotechnology , dialysis , dilution , chemistry , chromatography , antibiotics , biology , medicine , surgery , immunology , physics , thermodynamics , antibody
A constant benzylpenicillin (penicillin G) concentration for determination of the MIC for strains of Borrelia burgdorferi was achieved by dialysis culture. The strains were grown in dialysis membrane bags with daily transfer to tubes with freshly added benzylpenicillin. The MICs decreased by one or several dilution steps compared with the conventional procedure.

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