Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology | Zendy

Brian L. Robbins | Zendy; Benjamin Waibel | Zendy; Arnold Fridland | Zendy

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Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology

Author(s) -

Brian L. Robbins,

Benjamin Waibel,

Arnold Fridland

Publication year - 1996

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.40.11.2651

Subject(s) - zidovudine , radioimmunoassay , pharmacokinetics , cartridge , chromatography , pharmacology , detection limit , in vivo , drug , chemistry , human immunodeficiency virus (hiv) , medicine , virology , biochemistry , viral disease , biology , mechanical engineering , microbiology and biotechnology , engineering

This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug. This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV. The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations. The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.

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