TEM-28 from an Escherichia coli clinical isolate is a member of the His-164 family of TEM-1 extended-spectrum beta-lactamases | Zendy

Patricia A. Bradford | Zendy; N V Jacobus | Zendy; Niraja Bhachech | Zendy; Karen Bush | Zendy

Open Access

TEM-28 from an Escherichia coli clinical isolate is a member of the His-164 family of TEM-1 extended-spectrum beta-lactamases

Author(s) -

Patricia A. Bradford,

N V Jacobus,

Niraja Bhachech,

Karen Bush

Publication year - 1996

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.40.1.260

Subject(s) - aztreonam , cefotaxime , escherichia coli , ceftazidime , benzylpenicillin , beta lactamase , nucleotide , microbiology and biotechnology , enterobacteriaceae , biology , antibiotics , chemistry , bacteria , biochemistry , genetics , gene , penicillin , pseudomonas aeruginosa

TEM-28 (pI 6.1), expressed by an Escherichia coli clinical isolate, is a novel beta-lactamase which hydrolyzed ceftazidime, cefotaxime, and aztreonam with rates of 25, 1.1, and 5.6, respectively, relative to that for benzylpenicillin (100). The nucleotide sequence of blaTEM-28 differed from that of blaTEM-1 by two base changes, resulting in amino acid substitutions of Arg-164 to His and Glu-240 to Lys.

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