Variables Influencing Susceptibility Testing of Cryptococcus neoformans to 5-Fluorocytosine | Zendy

Edward R. Block | Zendy; Anne Jennings | Zendy; John E. Bennett | Zendy

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Variables Influencing Susceptibility Testing of Cryptococcus neoformans to 5-Fluorocytosine

Author(s) -

Edward R. Block,

Anne Jennings,

John E. Bennett

Publication year - 1973

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.4.4.392

Subject(s) - cryptococcus neoformans , minimum inhibitory concentration , microbiology and biotechnology , incubation , cryptococcus , dilution , serial dilution , incubation period , biology , antibiotics , medicine , pathology , biochemistry , physics , alternative medicine , thermodynamics

The minimum inhibitory concentration (MIC) of 5-fluorocytosine (5-FC) was determined for 65 isolates of Cryptococcus neoformans by using a twofold serial tube dilution method. The MIC was profoundly influenced by incubation temperature, inoculum size, and duration of incubation. By using a standard set of test conditions, 100% of 49 pretreatment isolates of C. neoformans were susceptible to 10 mug of 5-FC per ml or less, and 9 (56%) of 16 isolates recovered during or after 5-FC therapy were massively drug resistant (MIC > 320 mug/ml). With the standard test conditions recommended here, the tube dilution method was found to be both accurate and reproducible, and the results correlated with the treatment status of patients.

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