Reconstitution of Colicin E 2 -Induced Deoxyribonucleic Acid Degradation in Spheroplast Preparations | Zendy

R. Almendinger | Zendy; Lowell P. Hager | Zendy

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Reconstitution of Colicin E ₂ -Induced Deoxyribonucleic Acid Degradation in Spheroplast Preparations

Author(s) -

R. Almendinger,

Lowell P. Hager

Publication year - 1973

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.4.2.167

Subject(s) - colicin , spheroplast , endonuclease , deoxyribonuclease , ribonuclease , pancreatic ribonuclease , dna , biology , biochemistry , microbiology and biotechnology , autolysis (biology) , cytoplasm , deoxyribonucleases , enzyme , rna , plasmid , escherichia coli , gene

Spheroplasts are insensitive to colicin E(2) and do not show deoxyribonucleic acid (DNA) degradation even in the presence of massive amounts of E(2). However, when both endonuclease I and E(2) were present, spheroplast DNA was degraded by an endonucleolytic activity which gave rise primarily to double-strand DNA cleavages, producing fragments having an average molecular weight of 9 x 10(6). Pancreatic ribonuclease could substitute for colicin E(2) in the reconstitution system, but pancreatic deoxyribonuclease could not replace endonuclease I. However, colicin E(2) could not activate transfer ribonucleic acid-inhibited endonuclease I in an in vitro system where pancreatic ribonuclease caused full stimulation.

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