Open AccessIdentification of a carbenicillin-hydrolyzing beta-lactamase in Alcaligenes denitrificans subsp. xylosoxydans
Author(s) -
Dominique Decré,
G. Arlet,
E Bergogne-Bérézin,
A. Philippon
Publication year - 1995
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.39.3.771
Subject(s) - alcaligenes , carbenicillin , biology , microbiology and biotechnology , genomic dna , restriction enzyme , beta lactamase , gene , genetics , bacteria , escherichia coli , pseudomonas , antibiotics , ampicillin
Eleven strains of Alcaligenes denitrificans subsp. xylosoxydans produced a beta-lactamase with a pI of 5.7 with kinetic data characteristic of a PSE-1-type enzyme. A CARB-type enzyme was identified by using an intragenic DNA probe of blaCARB. Hybridization of genomic DNA after XbaI restriction and pulsed-field electrophoresis suggested a chromosomal location for the gene.