Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli | Zendy

Thomas W. North | Zendy; Gerd Hansen | Zendy; Yanqiu Zhu | Zendy; Judith A. Griffin | Zendy; C K Shih | Zendy

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Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli

Author(s) -

Thomas W. North,

Gerd Hansen,

Yanqiu Zhu,

Judith A. Griffin,

C K Shih

Publication year - 1994

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.38.2.388

Subject(s) - reverse transcriptase , feline immunodeficiency virus , escherichia coli , virology , biology , recombinant dna , nucleotidyltransferase , virus , rna directed dna polymerase , microbiology and biotechnology , lentivirus , rna , viral disease , gene , genetics

Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.

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