Open AccessLoss of function mutation in the yeast multiple drug resistance gene PDR5 causes a reduction in chloramphenicol efflux
Author(s) -
Pat Leonard,
P K Rathod,
John Golin
Publication year - 1994
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.38.10.2492
Subject(s) - biology , efflux , mutant , chloramphenicol , atp binding cassette transporter , yeast , saccharomyces cerevisiae , mutation , multiple drug resistance , gene , p glycoprotein , genetics , drug resistance , biochemistry , microbiology and biotechnology , antibiotics , transporter
The yeast (Saccharomyces cerevisiae) PDR5 gene product encodes a 160-kDa protein related to the large ABC family of transporters, including the human MDR1 multidrug resistance p-glycoprotein. Loss of function mutations in PDR5 result in chloramphenicol hypersensitivity. A pdr5::Tn5 loss of function mutant exhibits a markedly impaired efflux of chloramphenicol compared with that of an isogenic PDR5 (wild-type) control.