Open AccessContribution of the cell-surface-associated enzyme in the Zimmermann-Rosselet assay of outer membrane permeability of beta-lactam antibiotics
Author(s) -
W Liu,
Hiroshi Nikaido
Publication year - 1991
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.35.1.177
Subject(s) - bacterial outer membrane , lactam , enzyme , bacteria , membrane , permeability (electromagnetism) , beta lactam , antibiotics , chemistry , cell membrane , hydrolysis , membrane permeability , biochemistry , microbiology and biotechnology , biophysics , chromatography , biology , escherichia coli , stereochemistry , genetics , gene
Analysis of beta-lactam hydrolysis by intact cells of several strains of enteric bacteria suggests that a significant fraction of beta-lactamase molecules is present at a location directly accessible for the substrates in the medium. We propose a method to correct for the error introduced by these "surface-exposed" enzymes in the Zimmermann-Rosselet assay of outer membrane permeability.