Contribution of the cell-surface-associated enzyme in the Zimmermann-Rosselet assay of outer membrane permeability of beta-lactam antibiotics | Zendy

W Liu | Zendy; Hiroshi Nikaido | Zendy

Open Access

Contribution of the cell-surface-associated enzyme in the Zimmermann-Rosselet assay of outer membrane permeability of beta-lactam antibiotics

Author(s) -

W Liu,

Hiroshi Nikaido

Publication year - 1991

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.35.1.177

Subject(s) - bacterial outer membrane , lactam , enzyme , bacteria , membrane , permeability (electromagnetism) , beta lactam , antibiotics , chemistry , cell membrane , hydrolysis , membrane permeability , biochemistry , microbiology and biotechnology , biophysics , chromatography , biology , escherichia coli , stereochemistry , genetics , gene

Analysis of beta-lactam hydrolysis by intact cells of several strains of enteric bacteria suggests that a significant fraction of beta-lactamase molecules is present at a location directly accessible for the substrates in the medium. We propose a method to correct for the error introduced by these "surface-exposed" enzymes in the Zimmermann-Rosselet assay of outer membrane permeability.

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