Open AccessAntibodies to nystatin demonstrate polyene sterol specificity and allow immunolabeling of sterols in Saccharomyces cerevisiae
Author(s) -
H M Walker-Caprioglio,
John M. MacKenzie,
Leo W. Parks
Publication year - 1989
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.33.12.2092
Subject(s) - nystatin , ergosterol , sterol , polyene , saccharomyces cerevisiae , cholestanol , polyclonal antibodies , biochemistry , amphotericin b , biology , microbiology and biotechnology , chemistry , bovine serum albumin , antibody , cholesterol , yeast , antibiotics , antifungal , immunology
Polyclonal antibodies elicited by injection into rabbits of a nystatin-bovine serum albumin conjugate were reactive with both nystatin and amphotericin B. Upon labeling of polyene-treated Saccharomyces cerevisiae sterol auxotrophs grown on various sterols, nystatin reacted specifically with ergosterol, while amphotericin B did not react preferentially with ergosterol, cholesterol, or cholestanol. Time course labeling experiments demonstrated the rate of ergosterol transport into cholesterol-grown cells.