Open AccessHigh-level resistance to trimethoprim in Shigella sonnei associated with plasmid-encoded dihydrofolate reductase type I
Author(s) -
Rafaël Delgado,
Joaquín R. Otero
Publication year - 1988
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.32.10.1598
Subject(s) - shigella sonnei , plasmid , dihydrofolate reductase , biology , shigella , microbiology and biotechnology , trimethoprim , gene , enterobacteriaceae , escherichia coli , genetics , virology , antibiotics
By DNA hybridization, the gene encoding dihydrofolate reductase type I was found in 58 of 59 highly trimethoprim-resistant clinical isolates of Shigella sonnei obtained from 1981 through 1987 in Madrid, Spain. No strain harbored the type II gene. In selected strains, the type I gene was demonstrated to be in a plasmid.