Open AccessTurbidimetric Bioassay for Carbenicillin
Author(s) -
J P Stankewich,
Ronald P Upton
Publication year - 1973
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.3.3.364
Subject(s) - carbenicillin , bioassay , escherichia coli , microbiology and biotechnology , chemistry , chromatography , biology , antibiotics , biochemistry , ampicillin , genetics , gene
A quantitative turbidimetric bioassay has been developed for carbenicillin with the use of a strain ofEscherichia coli . The assay is relatively specific for carbenicillin and is not affected by 10% (wt/wt) benzylpenicillin. It has been demonstrated to be more precise than the plate diffusion assay. Since it is readily automated, it is also faster and less expensive. The influence of pH, time, and extent of growth on the assay has been evaluated.