Cytotoxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse tumor cells | Zendy

M J Sheu | Zendy; W W Baldwin | Zendy; Kenneth W. Brunson | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cytotoxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse tumor cells

Author(s) -

M J Sheu,

W W Baldwin,

Kenneth W. Brunson

Publication year - 1985

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.28.5.626

Subject(s) - cytotoxicity , fibroblast , cell culture , cytotoxic t cell , biology , microbiology and biotechnology , ratón , connective tissue , cell , macrophage , in vitro , biochemistry , immunology , genetics

The cytotoxicity of cationic peptides MCP-1 and MCP-2 isolated from rabbit alveolar macrophages was tested against two tumor cell lines of murine lymphosarcoma origin, RAW117-P and RAW117-H10, and a normal mouse connective tissue fibroblast strain, ATCC CCL1.RAW117-H10 is a highly malignant metastatic variant derived from the less malignant RAW117-P. Our results indicate that these peptides possess a cytotoxic activity against the tumor cells tested but not against the normal cells tested. At concentrations of 30 micrograms/ml, these peptides completely killed RAW117-H10 cells in suspension cultures, while inhibiting growth of RAW117-P for but a limited period of time, up to 48 h, after which growth resumed. RAW117-P cells were killed by concentrations of 40 micrograms/ml. These peptides showed little cytotoxicity for normal mouse connective tissue fibroblasts at concentrations of 40 micrograms/ml.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research