A gene probe for TEM type beta-lactamases | Zendy

Robert C. Cooksey | Zendy; Nancye C. Clark | Zendy; C Thornsberry | Zendy

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A gene probe for TEM type beta-lactamases

Author(s) -

Robert C. Cooksey,

Nancye C. Clark,

C Thornsberry

Publication year - 1985

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.28.1.154

Subject(s) - ecori , pbr322 , plasmid , biology , agarose , nucleic acid , gene , microbiology and biotechnology , restriction fragment length polymorphism , genetics , dna , structural gene , restriction enzyme , polymerase chain reaction , escherichia coli

A restriction fragment of plasmid pBR322 bearing the TEM-1 beta-lactamase structural gene was electroeluted from agarose gels after digestion with EcoRI and HinfI. The 1-kilobase fragment was 32P-labeled and used to examine genetic relationships with nucleic acids encoding seven other beta-lactamase classes. The probe hybridized only with TEM-2 and OXA-2 class plasmids.

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