Identification of small DNA fragments synthesized in herpes simplex virus-infected cells in the presence of acyclovir | Zendy

Paul V. McGuirt | Zendy; James E. Shaw | Zendy; Gertrude B. Elion | Zendy; P A Furman | Zendy

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Identification of small DNA fragments synthesized in herpes simplex virus-infected cells in the presence of acyclovir

Author(s) -

Paul V. McGuirt,

James E. Shaw,

Gertrude B. Elion,

P A Furman

Publication year - 1984

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.25.4.507

Subject(s) - herpes simplex virus , dna , biology , virology , virus , microbiology and biotechnology , thymidine , polymerase chain reaction , centrifugation , dna polymerase , herpesviridae , gene , viral disease , biochemistry

The effect of acyclovir on DNA synthesized in cells infected with herpes simplex virus type 1 was examined. DNA that was synthesized in infected cells in the presence of acyclovir during a short pulse with [3H]thymidine remained near the top of an alkaline sucrose gradient after centrifugation. The sedimentation characteristics of labeled DNA were not changed after chasing in isotope-free medium. The slowly sedimenting DNA was identified as viral in origin by hybridization to purified herpes simplex virus nucleocapsid DNA. When cells were infected with acyclovir-resistant virus containing mutations in the polymerase gene, the viral DNA synthesized in the presence of acyclovir was chased into high-molecular-weight DNA. These findings are consistent with chain termination of herpes simplex virus DNA in virus-infected cells.

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