Open AccessEstablishment of a murine cell line resistant to arabinosyladenine and devoid of adenosine deaminase activity
Author(s) -
Charles Shipman,
Shiu-Lok Tong,
Sandra H. Smith,
N. B. Katlama,
John C. Drach
Publication year - 1983
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.24.6.947
Subject(s) - adenosine deaminase , cell culture , adenosine , population , adenosine kinase , microbiology and biotechnology , substrate specificity , biology , chemistry , enzyme , biochemistry , medicine , genetics , environmental health
A population of cells stably resistant to a concentration of arabinosyladenine 100-fold greater than that tolerated by wild-type cells was selected over a period of 15 months. The cells are not cross resistant to arabinosylcytosine and may have at least one kinase with altered substrate specificity.