Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli | Zendy

R.Nagaraja Rao | Zendy; Norris E. Allen | Zendy; J N Hobbs | Zendy; William E. Alborn | Zendy; Herbert A. Kirst | Zendy; Jonathan W. Paschal | Zendy

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Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli

Author(s) -

R.Nagaraja Rao,

Norris E. Allen,

J N Hobbs,

William E. Alborn,

Herbert A. Kirst,

Jonathan W. Paschal

Publication year - 1983

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.24.5.689

Subject(s) - hygromycin b , escherichia coli , aminocyclitol , plasmid , phosphotransferase , biology , neomycin , microbiology and biotechnology , gene , aminoglycoside , biochemistry , antibiotics

A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The specific modification of hygromycin B is a phosphorylation of the hydroxyl on the 4 position of the cyclitol ring (hyosamine). The presence of the phosphotransferase in E. coli correlates with reduced accumulation of [14C]hygromycin B.

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