Determination of cefoperazone concentration in serum and muscle tissue with a versatile high-pressure liquid chromatographic method | Zendy

Robert R. Muder | Zendy; Warren F. Diven | Zendy; Victor L. Yu | Zendy; Jenifer L. Johnson | Zendy

Open Access

Determination of cefoperazone concentration in serum and muscle tissue with a versatile high-pressure liquid chromatographic method

Author(s) -

Robert R. Muder,

Warren F. Diven,

Victor L. Yu,

Jenifer L. Johnson

Publication year - 1982

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.22.6.1076

Subject(s) - chromatography , cefoxitin , cefoperazone , moxalactam , chemistry , acetonitrile , coefficient of variation , extraction (chemistry) , methanol , high performance liquid chromatography , cephalosporin , antibiotics , biology , bacteria , biochemistry , organic chemistry , antibiotic resistance , imipenem , genetics , staphylococcus aureus

A rapid, specific, and reproducible high-pressure liquid chromatographic method was developed for the determination of cefoperazone concentration in serum and tissue. The assay uses a simple methanol extraction, with cefoxitin as the internal standard. The limits of detection are 1 to 150 micrograms/ml; the maximum coefficient of variation is 7.4%. Using the same chromatography column, muBondapak phenyl, and mobile-phase 0.005 M tetrabutylammonium buffer-acetonitrile (80:20), the method can be easily adapted for the analysis of cefoxitin and moxalactam.

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