Fluorimetric determination of cefuroxime in body fluids

A simple and accurate fluorimetric procedure for measuring cefuroxime in body fluids is described. A fluorescent product was produced by addition of hydrochloric acid, heating, and cooling, followed by addition of sodium hydroxide and further heating at 100 degree C. The fluorescence intensity of the final solution was measured in a fluorimeter at an excitation wavelength of 375 nm and an emission wavelength of 440 nm and related to the antibiotic concentration. Thin-layer chromatography of the final solution showed a single fluorescent spot (Rf value, 0.6). Freedom from interference from other therapeutic agents and endogenous substances, as well as the close correlation between this method and the standard microbiological assay method, was demonstrated. The simplicity and reliability of the fluorimetric assay method make it particularly suitable for clinical use.