Comparison of enzymatic and liquid chromatographic chloramphenicol assays | Zendy

Allan Weber | Zendy; K E Opheim | Zendy; Jeffrey R. Koup | Zendy; Arnold L. Smith | Zendy

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Comparison of enzymatic and liquid chromatographic chloramphenicol assays

Author(s) -

Allan Weber,

K E Opheim,

Jeffrey R. Koup,

Arnold L. Smith

Publication year - 1981

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.19.2.323

Subject(s) - chloramphenicol , chromatography , chemistry , linear regression , enzyme , antibiotics , biochemistry , mathematics , statistics

A radioenzymatic assay and a "high-performance" liquid chromatographic assay for chloramphenicol were compared by using 52 patient specimens, 24 mock unknowns, and 13 quality control samples. Both methods were found to be rapid, precise, accurate, and sensitive, and either would be suitable for monitoring chloramphenicol concentrations in small volumes of serum. Linear regression analysis of serum chloramphenicol concentrations in patients receiving chloramphenicol succinate yielded a regression equation of Y = 1.04X + 0.274 (X = high-performance liquid chromatographic assay; Y = radioenzymatic assay), with a correlation coefficient of 0.971.

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