High-pressure liquid chromatographic method for determination of rosaramicin in humans

A high-pressure liquid chromatographic method has been developed for the quantitative determination of rosaramicin in serum. This procedure involves addition of an internal standard, adjustment to alkaline pH, treatment with potassium carbonate, ether extraction, and a reverse-phase column separation with acetonitrile-acetate buffer mixture as the mobile phase. This technique produces a good linear relationship between the peak height ratio and the rosaramicin concentration. In addition, this method has proven to be quite specific for rosaramicin, since many of its derivatives tested do not interfere with the assay. The method is accurate and reproducible with a sensitivity of about 0.01 microgram of rosaramicin per ml of serum. It may be useful in monitoring drug levels in serum of patients and also for the pharmacokinetic studies of the drug in humans.