Open AccessEffects of Fildes Enrichment on Antibiotic Susceptibility Testing of Haemophilus influenzae in the Autobac
Author(s) -
David Robinson,
Ronald F. Schell,
Jack L. LeFrock,
John Chan
Publication year - 1979
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.16.1.98
Subject(s) - haemophilus influenzae , ampicillin , microbiology and biotechnology , tetracycline , chloramphenicol , minimum inhibitory concentration , agar dilution , chemistry , haemophilus , antibiotics , agar dilution method , biology , bacteria , genetics
The results of this investigation showed that the Autobac could be used to determine the susceptibility ofHaemophilus influenzae to ampicillin, chloramphenicol, and tetracycline when eugonic broth was supplemented with 1.5% Fildes enrichment. An excellent correlation was obtained between the susceptibilities as determined by the agar dilution minimal inhibitory concentration method and the Autobac. For ampicillin allH. influenzae isolates with minimal inhibitory concentrations of ≤1 μg/ml had light scattering indexes of ≥0.60. Those isolates with minimal inhibitory concentrations of ≥4 μg/ml had light scattering indexes of ≤0.49. For chloramphenicol and tetracycline all isolates had minimal inhibitory concentrations of ≤1 μg/ml and light scattering indexes of ≥0.71.