Purification of a Streptococcal Bacteriocin (Viridin B) and Its Separation from Alpha-Hemolysin | Zendy

L D Apelgren | Zendy; Adnan S. Dajani | Zendy

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Purification of a Streptococcal Bacteriocin (Viridin B) and Its Separation from Alpha-Hemolysin

Author(s) -

L D Apelgren,

Adnan S. Dajani

Publication year - 1979

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.15.3.436

Subject(s) - bacteriocin , sephadex , hemolysin , ammonium sulfate precipitation , size exclusion chromatography , chromatography , microbiology and biotechnology , polyacrylamide gel electrophoresis , chemistry , biology , biochemistry , antimicrobial , virulence , gene , enzyme

Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.

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