High-Performance Liquid Chromatographic Assay of Chloramphenicol in Serum | Zendy

Jeffrey R. Koup | Zendy; Barbara Brodsky | Zendy; Alan H. Lau | Zendy; Thomas R. Beam | Zendy

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High-Performance Liquid Chromatographic Assay of Chloramphenicol in Serum

Author(s) -

Jeffrey R. Koup,

Barbara Brodsky,

Alan H. Lau,

Thomas R. Beam

Publication year - 1978

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.14.3.439

Subject(s) - chromatography , high performance liquid chromatography , chloramphenicol , chemistry , extraction (chemistry) , absorbance , acetonitrile , ethyl acetate , reversed phase chromatography , quantitative analysis (chemistry) , antibiotics , biochemistry

A new method for the analysis of serum chloramphenicol by reversed-phase, high-performance liquid chromatography (HPLC) is described. The method involves a preliminary extraction of 0.1 ml of serum with ethyl acetate containing an internal standard, chromatography with a reversed-phase C18 microparticulate column with an acetonitrile-acetate buffer mobile phase, and detection by measuring UV absorbance at 270 nm. Assay performance was compared with an existing microbiological assay. The HPLC method demonstrated both increased precision and increased sensitivity. The specificity of the HPLC method was also evaluated. The new method presents an alternative approach to the analysis of clinical specimens.

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