Open AccessAntibiotic Biosynthesis by Cofermentation of Blocked Mutants of Two Micromonospora Species
Author(s) -
B. K. Lee,
T. L. Nagabhushan,
R. G. Condon,
A. B. Cooper,
J. A. Waitz
Publication year - 1978
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.14.1.73
Subject(s) - micromonospora , aminocyclitol , antibiotics , mutant , biosynthesis , aminoglycoside , microbiology and biotechnology , biology , biochemistry , stereochemistry , chemistry , bacteria , genetics , gene , streptomyces
Two aminocyclitol-negativeMicromonospora mutants representing two different species,M. purpurea andM. inyoensis , and blocked at different steps in the biosynthetic pathway were paired and cofermented for the synthesis of antibiotics. The two blocked mutants were incapable of producing antibiotics alone except when 2-deoxystreptamine was added. When combined they produced gentamicins A, X2 , C1a , and C2b , which all have an amino group at the 2′ position, and gentamicin B, which has a hydroxyl group at this position instead.