Iodometric Assay Method for Beta-Lactamase with Various Beta-Lactam Antibiotics as Substrates | Zendy

Tetsuo Sawai | Zendy; Ikuko Takahashi | Zendy; Saburo Yamagishi | Zendy

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Iodometric Assay Method for Beta-Lactamase with Various Beta-Lactam Antibiotics as Substrates

Author(s) -

Tetsuo Sawai,

Ikuko Takahashi,

Saburo Yamagishi

Publication year - 1978

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.13.6.910

Subject(s) - cefazolin , carbenicillin , cephaloridine , cephalosporin , cefoxitin , cloxacillin , cefalexin , chemistry , cefalotin , ampicillin , beta lactamase , benzylpenicillin , hydrolysis , antibiotics , cephalosporin antibiotic , chromatography , microbiology and biotechnology , penicillin , biochemistry , staphylococcus aureus , bacteria , biology , escherichia coli , gene , genetics

The rapid fixed-time assay for penicillinase was modified for measuring beta-lactamase activity with twelve substrates, i.e., benzylpenicillin, ampicillin, cloxacillin, methicillin, carbenicillin, cefazolin, cephalothin, cephaloglycin, cephalexin, cephalosporin C, 7-aminocephalosporanic acid, and cefoxitin. The method depends upon the reduction of iodine by the hydrolyzed substrate. Determined experimentally, 1 mol of hydrolyzed penicillins consumed 3.4 to 4.0 mol of iodine (I(2)). Iodine consumption of hydrolyzed cephalosporins varied widely from 1.7 for cephalothin to 3.7 for cefazolin. The method is useful for routine assay of beta-lactamase activity with various substrates.

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