Open AccessMicrobial Degradation of Cephalothin by Cephalothin-Susceptible Escherichia coli
Author(s) -
Tsuneo Nishiura,
Yukimichi Kawada,
Yoko Shiomi,
Koji Ohara,
Megumi Kono
Publication year - 1978
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.13.6.1036
Subject(s) - escherichia coli , cephalosporin , degradation (telecommunications) , incubation , microbiology and biotechnology , enterobacteriaceae , chemistry , substrate (aquarium) , enzyme , bacteria , cephalosporin antibiotic , chromatography , biochemistry , antibiotics , biology , telecommunications , ecology , genetics , computer science , gene
Cephalothin (CET)-susceptibleEscherichia coli , which can degrade CET after prolonged incubation in broth containing a concentration of the drug greater than the minimum inhibitory concentration, was found in a clinical specimen. The substrate specificity of the partially purified enzyme to cephalosporin analogs strongly indicated the occurrence of CET-specific degradation. Nuclear magnetic resonance analysis of the degradation reaction demonstrated the appearance of two new signals attributed to deacetyl CET. This suggests the possibility of the presence of acylesterase.