Restriction Enzyme Analysis of Plasmids from Haemophilus influenzae | Zendy

N. K. Harkess | Zendy; Michael L. Murray | Zendy

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Restriction Enzyme Analysis of Plasmids from Haemophilus influenzae

Author(s) -

N. K. Harkess,

Michael L. Murray

Publication year - 1978

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.13.5.802

Subject(s) - plasmid , ampicillin , haemophilus influenzae , agarose gel electrophoresis , biology , microbiology and biotechnology , molecular mass , restriction enzyme , gel electrophoresis , agarose , amp resistance , escherichia coli , enzyme , gene , genetics , biochemistry , antibiotics

Examination of Haemophilus influenzae strains isolated in New Orleans revealed ampicillin-resistant strains with plasmids of size classes not previously detected in North America. The molecular weight of plasmids in five ampicillin-resistant strains ranged from 0.8 x 10(6) daltons (0.8 Mdal) to 36 Mdal. The molecular weights of the plasmids were determined by sucrose gradient centrifugation, electron microscopy, and agarose gel electrophoresis. Plasmids of the previously detected 30-Mdal size class were found in three of the five ampicillin-resistant strains. Restriction enzyme analysis is consistent with a close relationship between these three 30-Mdal plasmids. Of the two remaining ampicillin-resistant strains, one contained a single plasmid of 36 Mdal and the other contained two plasmids of 0.8 and 2.3 Mdal.

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