Open Access5,6-Dihydro-5-Azathymidine: In Vitro Antiviral Properties Against Human Herpesviruses
Author(s) -
Harold E. Renis
Publication year - 1978
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.13.4.613
Subject(s) - herpes simplex virus , virus , deoxyuridine , virology , idoxuridine , varicella zoster virus , nucleoside , pseudorabies , vaccinia , herpesviridae , thymidine , nucleoside analogue , simplexvirus , virus quantification , vidarabine , in vitro , biology , chemistry , biochemistry , chemotherapy , viral disease , dna , genetics , fludarabine , gene , recombinant dna , cyclophosphamide
5,6-Dihydro-5-azathymidine (DHAdT), a novel water-soluble nucleoside antibiotic, inhibits herpes simplex virus type 1 (HSV-1) in appropriately infected cell cultures to a greater extent than herpes simplex virus type 2 (HSV-2). Vaccinia virus was less susceptible than HSV-2, and pseudorabies virus yields were not reduced at the concentrations studied. Plaque formation by varicella-zoster virus was suppressed by DHAdT. DHAdT was slightly toxic to cells at concentrations that were inhibitory for HSV-1 and varicella-zoster virus. Thymidine and deoxyuridine completely reversed the anti-HSV-1 activity of DHAdT, whereas deoxycytidine was partially effective. Compared with other nucleoside analogs with activity for HSV-1, DHAdT was less active than 5-iodo-2′-deoxyuridine or 1-β-d -arabinofuranosylcytosine and nearly equipotent with 9-β-d -arabinofuranosyladenine.