Open AccessInterferon Synthesis in Human Diploid Cells Pretreated with Insulin
Author(s) -
Mitsuo Azuma,
Tohru Suenaga,
Itsuro Yoshida,
Fumio Mizuno
Publication year - 1978
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.13.4.566
Subject(s) - insulin , interferon , medicine , endocrinology , wi 38 , biology , human insulin , newcastle disease , virus , ploidy , virology , biochemistry , gene
Interferon production by Newcastle disease virus in human diploid (WI-38) cells was greatly reduced in the absence of serum. Pretreatment of the serum-free cells with insulin in excess of 1.2 IU/ml for 2 h before induction considerably increased the amount of interferon produced. No enhancement of interferon production was seen when the cells were exposed to insulin 2 h after induction or later. The insulin's effect persisted for at least 6 h after its removal, but not for 24 h. A possible mechanism of insulin action is discussed.