Actinomycin Biosynthesis by Protoplasts Derived from Streptomyces parvulus | Zendy

Michael J. M. Hitchcock | Zendy; Edward Katz | Zendy

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Actinomycin Biosynthesis by Protoplasts Derived from Streptomyces parvulus

Author(s) -

Michael J. M. Hitchcock,

Edward Katz

Publication year - 1978

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.13.1.104

Subject(s) - mycelium , protoplast , sarcosine , valine , biosynthesis , methionine , biochemistry , biology , amino acid , glycine , streptomycetaceae , streptomyces , isoleucine , tryptophan , in vivo , actinomycetales , leucine , bacteria , botany , gene , genetics

Conditions are described for the formation of protoplasts from Streptomyces parvulus that are able to synthesize actinomycin D de novo. Antibiotic synthesis by protoplasts, in contrast to that by mycelium, was sensitive to inhibition by actinomycin D and to a decrease in sucrose concentration. On the other hand, synthesis by mycelium was much more sensitive to inhibition by amino acid analogs (d-valine, cis-3-methylproline, and alpha-methyl-dl-tryptophan). In addition, the uptake of amino acids (l-methionine, sarcosine, and l- and d-valine) by protoplasts was significantly lower than that by mycelium. The advantages and limitations of using protoplasts for studying in vivo actinomycin synthesis are discussed.

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