Open AccessRadiometric Determination of the Concentration of Amphotericin B in Body Fluids
Author(s) -
Roy L. Hopfer,
Dieter Gröschel
Publication year - 1977
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.12.6.733
Subject(s) - amphotericin b , candida albicans , paecilomyces , microbiology and biotechnology , agar , chemistry , antibiotics , incubation , biology , antifungal , biochemistry , bacteria , botany , genetics
A new assay was developed to determine the concentration of amphotericin B in body fluids. The Bactec radiometric system was used to measure CO2 production by a test strain,Candida albicans MDA 448, in the presence of amphotericin B. After 5-h incubation, drug concentrations as low as 0.2 μg/ml could be detected. The results are comparable to those of the commonly used agar diffusion assay withPaecilomyces varioti .