In Vitro Uptake of Gentamicin by Rat Renal Cortical Tissue | Zendy

Chen H. Hsu | Zendy; Theodore W. Kurtz | Zendy; John M. Weller | Zendy

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In Vitro Uptake of Gentamicin by Rat Renal Cortical Tissue

Author(s) -

Chen H. Hsu,

Theodore W. Kurtz,

John M. Weller

Publication year - 1977

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.12.2.192

Subject(s) - gentamicin , incubation , in vitro , tetraethylammonium chloride , chemistry , aminoglycoside , kidney , tetraethylammonium , dinitrophenol , in vivo , pharmacology , endocrinology , biochemistry , biology , antibiotics , potassium , microbiology and biotechnology , organic chemistry

The mechanism of gentamicin uptake in vitro by renal cortical slices of rat kidney was investigated. The cortical-slice-uptake ratio of gentamicin concentration in 1.0 g of tissue water to that of 1.0 ml of incubation medium (SW/M) was 1.44 +/- 0.04. The uptake of gentamicin was inhibited by 2 x 10(-5) M dinitrophenol (SW/M = 1.03 +/- 0.04) and by anoxia (SW/M = 1.01 +/- 0.04). The results indicate that aerobic phosphorylation is required to transport gentamicin into the cells. The uptake of p-aminohippurate and tetraethylammonium chloride by renal cortical slices was not affected by gentamicin.

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