Open AccessCyclic Adenylic Acid-Dependent and -Independent Production of Chloramphenicol Acetyltransferase in Escherichia coli Carrying R Plasmids
Author(s) -
Takeshi Yokota,
Rie Kuwahara,
Sumiko Hagiwara,
Shōgo Kuwahara
Publication year - 1977
Publication title -
antimicrobial agents and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.07
H-Index - 259
eISSN - 1070-6283
pISSN - 0066-4804
DOI - 10.1128/aac.11.6.952
Subject(s) - chloramphenicol acetyltransferase , plasmid , escherichia coli , chloramphenicol , biology , microbiology and biotechnology , acetyltransferase , vibrio cholerae , enterobacteriaceae , mutant , bacteria , antibiotics , gene , reporter gene , biochemistry , genetics , gene expression , acetylation
A plasmid-specified, inducible, but cyclic adenylic acid (cAMP)-independent resistance to chloramphenicol (CM) is reported. The resistance level to CM was increased two- to fourfold by treatment with a small amount of CM for a short period inEscherichia coli strains carrying the R plasmid pJY1, which was obtained from a clinical isolate ofVibrio cholerae . Though cAMP was required for production of CM acetyltransferase (CATase) in cAMP-deficient mutants ofE. coli carrying the R100 plasmid, the same species harboring pJY1 did not require cAMP for production of the enzyme. The possibility of a mechanism other than CATase activity for the CM resistance conferred by R plasmids is discussed.