Cyclic Adenylic Acid-Dependent and -Independent Production of Chloramphenicol Acetyltransferase in Escherichia coli Carrying R Plasmids | Zendy

Takeshi Yokota | Zendy; Rie Kuwahara | Zendy; Sumiko Hagiwara | Zendy; Shōgo Kuwahara | Zendy

Open Access

Cyclic Adenylic Acid-Dependent and -Independent Production of Chloramphenicol Acetyltransferase in Escherichia coli Carrying R Plasmids

Author(s) -

Takeshi Yokota,

Rie Kuwahara,

Sumiko Hagiwara,

Shōgo Kuwahara

Publication year - 1977

Publication title -

antimicrobial agents and chemotherapy

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.07

H-Index - 259

eISSN - 1070-6283

pISSN - 0066-4804

DOI - 10.1128/aac.11.6.952

Subject(s) - chloramphenicol acetyltransferase , plasmid , escherichia coli , chloramphenicol , biology , microbiology and biotechnology , acetyltransferase , vibrio cholerae , enterobacteriaceae , mutant , bacteria , antibiotics , gene , reporter gene , biochemistry , genetics , gene expression , acetylation

A plasmid-specified, inducible, but cyclic adenylic acid (cAMP)-independent resistance to chloramphenicol (CM) is reported. The resistance level to CM was increased two- to fourfold by treatment with a small amount of CM for a short period inEscherichia coli strains carrying the R plasmid pJY1, which was obtained from a clinical isolate ofVibrio cholerae . Though cAMP was required for production of CM acetyltransferase (CATase) in cAMP-deficient mutants ofE. coli carrying the R100 plasmid, the same species harboring pJY1 did not require cAMP for production of the enzyme. The possibility of a mechanism other than CATase activity for the CM resistance conferred by R plasmids is discussed.

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